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yxeM [2018-03-05 09:27:25]
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yxeM [2018-03-05 09:27:25]

putative cysteine ABC transporter (binding protein), the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
locus
BSU39500
pI
9.09
mw
29.16 kDa
protein length
264 aa Sequence Blast
gene length
792 bp Sequence Blast
function
uptake of cysteine
product
putative cysteine ABC transporter (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    4,059,488 → 4,060,282

    The protein

    Protein family

  • bacterial solute-binding protein 3 family (according to Swiss-Prot)

    • [SW|Localization]

  • associated to the membrane (via [protein|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|YxeN]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B782 (yxeM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1781 NBRP B. subtilis, Japan]
  • 1A950 ( ''yxeM''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
  • BKE39500 (Δ[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT, downstream forward: _UP4_TAAGAAACGGCGGTGACTCA
  • BKK39500 (Δ[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT, downstream forward: _UP4_TAAGAAACGGCGGTGACTCA

    • References

  • 10092453,12193636,16513748,10746760,21815947